Agar block technique for identification of mycoplasmas by use of fluorescent antibody.

Characterization of canine mycoplasmas by polyacrylamide gel electrophoresis and immunodiffusion.

Canine mycoplasmas which had been characterized by biological and serological methods were further studied by using polyacrylamide gel electrophoresis (PGE) and double diffusion in agar gel. The three dog mycoplasmas previously characterized, Mycoplasma canis, M. maculosum, and M. spumans showed distinctive patterns by PGE. Five additional representative isolates from dogs had been characterize...

Visualization of Horse Sickness virus by the Fluorescent antibody technique

The first report of Clostridium oedematiens infection in sheep in Iran by the use of Fluorescent Labelled Antibody

Using PCR and culture methods for Mycoplasma testing

Mycoplasma contaminants can be considered important not only for their roles as pathogens but also they may indicate that insufficient care has been taken during vaccine manufacture or quality control. The purpose of this study was to test the poliomyelitis vaccines for Mycoplasma by culture and polymerase chain reaction (PCR) methods. During 2008 to 2009, a total of 47 lots of oral poliomyelit...

Application of culture and polymerase chain reaction (PCR) methods for isolation and identification of Mycoplasma synoviae on broiler chicken farms

Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...